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STS-65
STS-65 spacelab.jpg
The Spacelab Module LM1 inside the Space Shuttle Columbia, used for the International Microgravity Laboratory
Mission type Microgravity research
Operator NASA
Mission duration 14 days, 17 hours, 55 minutes
Distance travelled 9,886,200 kilometers (6,143,000 mi)
Orbits completed 235
Spacecraft properties
Spacecraft Space Shuttle Columbia
Payload mass 10,811 kilograms (23,834 lb)
Crew
Crew size 7
Members
  • Robert D. Cabana
  • James D. Halsell
  • Richard J. Hieb
  • Carl E. Walz
  • Leroy Chiao
  • Donald A. Thomas
  • Chiaki Naito-Mukai
Start of mission
Launch date 8 July 1994, 16:43:01 (1994-07-08UTC16:43:01Z) UTC
Launch site Kennedy LC-39A
End of mission
Landing date 23 July 1994, 10:38:01 (1994-07-23UTC10:38:02Z) UTC
Landing site Kennedy SLF Runway 33
Orbital parameters
Reference system Geocentric
Regime Low Earth
Perigee 300 kilometres (190 mi)
Apogee 304 kilometres (189 mi)
Inclination 28.45 degrees
Period 90.5 minutes
Sts-65-patch.png Sts-65 crew.jpg
Left to right - Seated: Hieb, Cabana, Thomas; Standing: Chiao, Halsell, Naito-Mukai, Walz
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STS-65 was a Space Shuttle mission using the orbiter Columbia. It launched from Kennedy Space Center, Florida, on July 8, 1994. The main goal of this mission was to study how things behave in microgravity, which is a very weak gravity environment. This mission was part of the Space Shuttle program.

The commander of STS-65 was Robert D. Cabana. He later became the director of the Kennedy Space Center.

Meet the Astronauts: The STS-65 Crew

The STS-65 mission had a crew of seven astronauts. They were all important for carrying out the many science experiments.

Position Astronaut
Commander Robert D. Cabana
Third spaceflight
Pilot James D. Halsell
First spaceflight
Mission Specialist 1 Richard J. Hieb
Third and last spaceflight
Mission Specialist 2 Carl E. Walz
Second spaceflight
Mission Specialist 3 Leroy Chiao
First spaceflight
Mission Specialist 4 Donald A. Thomas
First spaceflight
Payload Specialist 1 Chiaki Mukai, NASDA
First spaceflight

One of the crew members was Chiaki Mukai. She was the first Japanese woman to travel into space.

Mission Goals: Studying Life in Space

STS-065 shuttle
STS-65 launch

The STS-65 mission carried the International Microgravity Laboratory (IML-2). This was the second time a Spacelab module was used to do research in microgravity. Microgravity means a very low gravity environment, like what astronauts experience in orbit.

The IML-2 mission was a big international effort. Scientists from many countries worked together. These included the European Space Agency (ESA), Canada, France, Germany, and Japan. They partnered with NASA to provide experiments for the mission.

The main focus of IML-2 was on two areas:

  • Microgravity science: This involved understanding how materials behave without strong gravity. Scientists hoped to create new materials that are hard to make on Earth.
  • Life sciences: This looked at how living things, like cells and organisms, are affected by space. It also helped understand health issues for astronauts in space.

The experiments used special equipment inside the Spacelab module. This equipment allowed scientists to study different aspects of life and materials in space.

Life Science Experiments: Animals and Plants

The STS-65 mission included several important life science experiments. These studies helped scientists learn how living things adapt to space.

Newt Experiment: How Gravity-Sensing Organs Develop

STS-65 fig6
The inner ear's balance organs, called the vestibular apparatus, help us know where we are in space. The otolith organs, which sense gravity, have tiny stones (otoliths) that move when your head does. This movement tells your brain about motion and balance.

One experiment studied Japanese Red-Bellied Newts. Scientists wanted to see how their gravity-sensing organs developed in space. These organs are in the inner ear of animals. They contain tiny calcium carbonate stones called otoliths. On Earth, gravity pulls on these stones, sending signals to the brain about balance.

The experiment aimed to find out if growing up in microgravity would change how these otoliths form. It also looked at how the cells and nerves connected to them would develop.

Jellyfish Experiment: Behavior and Development in Space

Another experiment focused on Moon Jellyfish (Aurelia aurita). Researchers observed how jellyfish behaved and developed in space. They watched how they swam and moved. They also studied how their gravity-sensing organs formed.

A key part of this experiment was to see if a small amount of artificial gravity could help prevent any negative effects of space travel on the jellyfish.

Daylily Experiment: Plant Growth in Microgravity

The PEMBSIS experiment studied daylily cells. Scientists wanted to know if spaceflight changed how daylily embryos grew. They also checked if cell division and chromosomes were affected by the space environment.

Life Science Equipment and Organisms

Organisms Studied

  • Newts: Both adult and young Japanese Red-Bellied Newts (Cynopus pyrrhogaster) were used. Young newts were chosen because their balance system develops during the mission time. Adults could lay eggs in space, allowing scientists to study embryos that grew in microgravity.
  • Jellyfish: Moon Jellyfish (Aurelia aurita) were the subjects. Both the stationary polyp stage and the free-swimming ephyra stage were studied.
  • Daylilies: The PEMBSIS experiment used daylily cells (Hemerocallis cv. Autumn Blaze).
Cynops pyrrhogaster NASA
A Japanese Red-Bellied Newt (Cynops pyrrhogaster).
Aurelia aurita NASA
A Jellyfish (Aurelia aurita).

Special Hardware Used

To keep the organisms safe and allow for experiments, special equipment was used:

  • Aquatic Animal Experiment Unit (AAEU): This unit, from Japan's space agency (NASDA), housed the newts. It kept them alive in water for over 19 days.
  • Nizemi: This German-made microscope and camera system could rotate slowly. It allowed scientists to watch jellyfish behavior at different levels of gravity.
  • Biorack: The European Space Agency's Biorack facility held the jellyfish.
  • Refrigerator/Incubator Module (R/IM): This unit kept samples at controlled temperatures, either cool or warm.
  • Plant Fixation Chambers (PFCs): These sealed containers held the daylily cells. They allowed scientists to add chemicals in space to "fix" the plant cells for later study.
R IM NASA
The Refrigerator/Incubator Module (R/IM) kept things cool or warm in the Shuttle.
ATR 4 NASA
The Ambient Temperature Recorder (ATR-4) recorded temperatures in different places.

Mission Operations: What Happened During the Flight

Before Launch

  • Daylily cell cultures were prepared about a week before the mission.
  • Newt eggs and adult newts were loaded into their water tanks about 36 hours before launch.
  • Jellyfish polyps were given a special solution to make them change into the ephyra form 24 hours before launch.
  • All these setups had matching "ground controls" on Earth. This allowed scientists to compare what happened in space to what happened on Earth.

During the Flight

  • Astronauts regularly checked on the newt eggs and adults. Sadly, two adult newts died during the mission.
  • Jellyfish were videotaped using the rotating microscope to see how they swam in different gravity levels. Some jellyfish were preserved in space for later study.
  • Some daylily cell cultures were also preserved in space using a chemical solution.

After Landing

  • The newts were recovered about six hours after landing. Some larvae were preserved, and others were studied to see how space affected their balance.
  • Living jellyfish were counted, photographed, and studied for their pulse rate and body parts.
  • Daylily cells were also photographed and preserved shortly after landing to check for changes in their cells and chromosomes.

Mission Results: What Scientists Learned

Newt Study Findings

The newt larvae that grew up in space developed at the same speed as those on Earth. However, the space-reared larvae had larger endolymphatic sacs (part of the inner ear) and more otoconia (the tiny stones) inside them. The otoconia also appeared much faster in the newts that grew in microgravity.

Jellyfish Study Findings

Jellyfish that developed in microgravity had more abnormal arm numbers compared to those that developed on Earth. Also, fewer of the space-grown jellyfish swam normally after landing. Interestingly, jellyfish polyps that budded (a type of reproduction) in space produced more buds and developed faster than those on Earth. This suggests that while development generally went well, some jellyfish were more sensitive to microgravity.

Daylily Cell Study Findings

Scientists found changes in the cells and chromosomes of daylily cells that were in space. Many cells from the space samples had two nuclei (binucleate cells), while all the control cells on Earth had only one nucleus.

Newts in Space

During the mission, two of the four adult newts died. The first death was thought to be due to stress. The second newt was found by astronaut Donald A. Thomas on July 17, 1994. This second death was called "peculiar" by a scientist on the ground. It was difficult to remove the dead newt from the tank because of weightlessness. The newts were Japanese Red-bellied Newts (Cynops pyrrhogaster).

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