Protocol (science) facts for kids

A protocol is like a detailed instruction manual for doing something important, especially in science and social science. It's a set of rules and steps that scientists follow to make sure their experiments or studies are done in the exact same way every time.

Imagine you're baking a cake. If you want it to turn out the same delicious way every time, you follow a recipe, right? A protocol is similar, but for scientific work. It helps other scientists get the same results if they follow the same steps. This is called replication.

Protocols also help experts check each other's work, which is called peer review. They include not just the steps, but also:

• What the study wants to find out.
• Why the experiment was designed a certain way.
• Why a certain number of samples were used.
• Safety rules.
• How results were figured out, including math and statistics.
• Rules for deciding which data to include or exclude, to avoid being unfair (this is called bias).

Protocols are also used in places like hospitals, factories, and testing labs. For example, a hospital has protocols for blood testing to make sure it's done safely and accurately every time.

In social science, a protocol can also be a detailed record of what happened during an observation. This could be watching how babies react to new things or how animals behave in their natural homes. These records help scientists find patterns and understand why things happen. They might be written notes or even videos and audio recordings.

Protocols for Experiments and Studies

In fields like environmental science and clinical research (which studies health and medicine), many people need to work together in a standard way. Any lab tests or experiments must be done ethically (meaning fairly and safely) and in a way that others can repeat the results. This is why strict protocols are so important.

These predefined protocols are a key part of rules like Good Laboratory Practice (GLP) and Good Clinical Practice (GCP). Protocols often include or refer to standard operating procedures (SOPs), which are general rules for how a lab operates. They might also mention laws and regulations that apply to the work.

Before a protocol is used, it usually needs to be approved by important people, like a lab director or an ethics committee. This makes sure everything is safe and proper. Even research funded by big organizations like the National Institutes of Health requires clear protocols.

Clinical Trial Protocols

In a clinical trial (a study testing new medicines or treatments), the protocol is designed very carefully. It protects the health of the people taking part and helps answer specific research questions.

A clinical trial protocol describes:

• What kind of people can join the trial.
• The schedule for tests, procedures, medicines, and how much medicine to give.
• How long the study will last.

People in a clinical trial are regularly checked by research staff to make sure they are healthy and to see if the treatment is safe and works well. Since 1996, most clinical trials are expected to follow the CONSORT Statement. This statement helps scientists design and report their protocols clearly. Even though it's for health and medicine, its ideas are useful for other types of experimental research too.

What Protocols Often Cover

Protocols usually explain many important things:

• Safety: Safety rules are a very important part of any protocol. They can be simple, like requiring safety goggles, or more complex, like how to safely handle dangerous chemicals or tiny germs. Protocols might also explain what to do if an accident happens.
• Procedures: This section details all the steps to follow. It includes not just the main experiment steps, but also how to avoid contamination, how to check and set up equipment, and how to write down everything that happens. If someone finds a mistake in these steps, it could mean the results aren't valid.
• Equipment Used: Protocols list all the equipment needed. They explain how to test and set up the equipment, including its specifications (like how precise it is) and its normal operating range. Environmental factors, like temperature or humidity, can sometimes affect results, so good protocols also say to record these.
• Reporting: A protocol tells you how to report the results. This includes describing the experiment's design, the protocol itself, and any environmental or equipment issues that might have affected the results. Clear reporting helps everyone understand the study.
• Calculations and Statistics: If an experiment produces numbers, the protocol will usually have detailed formulas for figuring out the results. It might also include instructions for preparing special liquids or solutions needed for the work. Methods for statistical analysis (using math to understand data) are often included to help interpret the information.
• Avoiding Bias: Many protocols include ways to prevent unfairness or mistakes in how results are understood.

* Measurement errors happen in all measurements. These can be small mistakes from the equipment or from using rounded numbers in calculations. * Sample bias is when the group of people or things you study isn't truly representative of the larger group you want to learn about. For example, a political poll would be best if it only asked people who are likely to vote. Web polls are often not scientific because anyone can answer, which can lead to bias. * Sample size is also important. If you only ask 10 people, your results might not be very accurate. Concepts like Standard deviation and variance help scientists understand how reliable their sample size is. * The placebo effect (when people feel better just because they think they're getting treatment, even if it's fake) and observer bias (when researchers accidentally influence results) often require blinding. This means some people in the study don't know who is getting the real treatment and who isn't. A control group (a group that doesn't get the treatment) is also used for comparison.

It's a good idea to publish a protocol before starting a study. This helps avoid doing the same research twice and makes the study more open and consistent.

Blinded Protocols

Sometimes, a protocol requires blinding to avoid bias. This means hiding certain information from people involved in the experiment. This could include the people taking part in the study, the researchers, the technicians, or the people analyzing the data.

For example, in a medical study, the patients might not know if they are getting the real medicine or a placebo (a fake medicine). This is called a single blind study. If both the patients and the doctors don't know, it's a double blind study. This helps make sure that people's expectations don't accidentally change the results.

Sometimes, blinding isn't possible or wouldn't be ethical (fair). A good clinical protocol makes sure blinding is as effective as possible within what's ethical and practical.

If someone finds out information that was supposed to be hidden during the experiment, it's called unblinding. If this happens before the study ends, it can cause errors because the bias that blinding was supposed to prevent might come back. Unblinding can happen, and it's important for studies to measure and report it. However, in reality, not many studies actually do this.

Scientists might have some freedom in how they set up blinding, but they usually need to explain their choices if they publish their results or submit them to a regulatory agency. If researchers know which data is "negative" (doesn't support their idea) during an experiment, they might sometimes try to find reasons to exclude it. This rarely happens with "positive" data.

See also

In Spanish: Protocolo de investigación para niños